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Available Instruments

Reserve staff time for assistance with Experiment Design, including Panel Design, or Data Analysis.

Room: 
B015

Analyzers

Abbott is a 3-laser, 17-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 8-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
1651 Page Mill
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
405nm, 488nm, 633nm

3-laser (Blue-Violet-Red) spectral cytometer capable of  analyzing >20-color panels

Model: 
Aurora
Room: 
B016
Sort: 
No
Maximum cells/second: 
35000
BSL-2: 
Yes
Colors: 
>20
Laser Excitations: 
488, 405, 640

LSRII is a 4-laser, 18-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 8-Violet, 2-Blue, 5-Green, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
B016A
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 532nm, 635nm, 405nm

LSRII.2 is a 5-laser, 18-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 6-Violet, 2-Blue, 4-Green, 3-UV, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
B016A
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm

LSRII.UV is a 5-laser, 18-color instrument compatible with the 96-well plate sampler.  It has recently been upgraded to support the latest violet and UV excited reagents.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 5-Yellow, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
B016D
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm

The FACScan Analyzer was one of the first successful bench top flow cytometers. They just keep going and going. This one has been upgraded to 4-laser, 12-color configuration.  Its current laser/detector configuration provides 2-Violet(co-linear with Red, 3-Red (co-linear with Violet), 3-Blue, and 4-Yellow laser excited detection channels.

Model: 
Custom FACScan
Room: 
B016D
Sort: 
No
Maximum cells/second: 
3,000
BSL-2: 
Yes
Colors: 
12
Laser Excitations: 
488nm, 405 nm, 561nm, 640nm

Mass Cytometer

The CyTOF2 Mass Cytometer uses metal-ion conjugates to label cells.  Labeled cells are then ionized and mass measurements are used to differentiate functional and phenotypic markers.  Sample preparation and processing is quite similar to that used in fluorescence-based cytometry with some quality control considerations unique to atomic mass spectroscopy. Please contact staff for further details.

Model: 
DVS CyTOF II
Room: 
B015A
Sort: 
No
Maximum cells/second: 
500
BSL-2: 
Yes
Colors: 
~100
Laser Excitations: 
N/A

Sorters

Aida

Aida is a 4-laser, 14-color sorter.  Its current laser/detector configuration provides 5-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
13
Laser Excitations: 
488nm, 561nm, 635nm, 405nm
Aria

Aria is a 3-laser, 11-color sorter.  Its current laser/detector configuration provides 3-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B016C
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
11
Laser Excitations: 
488nm, 635nm, 405nm
Carmen

Carmen is a 6 laser sorter capable of up to 6 way sorting.  It is our fastest plate sorter; both 384- and 96- well plate sorts require only 1/3 the time needed on an Aria type sorter.  It is the only instrument with an 457nm excitation laser, making it ideal for fluorochromes such as CFP or mTurquoise.  It is available only with operator support.

Model: 
BD Influx Special Order
Room: 
B015A
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
355nm, 405nm, 457nm, 488nm, 561nm, 640nm

Costello is a 5 laser, 18-color Aria Fusion sorter.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria Fusion
Room: 
1651 Page Mill
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm
Falstaff

Falstaff is a 5-laser, 17-color sorter.  Its current laser/detector configuration provides 2-UV, 5-Violet, 2-Blue, 5-Green, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm
Mikado

is the most automated sorter that the Facility has. It is simple to use, and has tray and idex sorting. It is the best sorter for 384 trays, It has 3 lasers (405 nm, 488nm, 561nm) but is a little limited in fluorochrome choices as these lasers are co-linear,

Model: 
Sony SH800s
Room: 
B016B
Sort: 
Yes
Maximum cells/second: 
7500
BSL-2: 
Yes
Colors: 
6
Laser Excitations: 
405nm,488nm,561 nm
PICI

PICI is a 5 laser, 18 color Aria Fusion sorter that was bought with Parker Institute for Cancer Immunotherapy funds.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria Fusion
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm
Satchmo

is a simple two-laser (488 nm and 640 nm) sorter. It is easy to operate, and does fast tray sorting, as well as index sorting. It uses the same polypropylene sample tubes as Carmen.

Model: 
BD FACSJazz
Room: 
B016B
Sort: 
Yes
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
6
Laser Excitations: 
488 nm, 640 nm
Vantoo

Vantoo is a 7 color 2 laser sorter upgraded with digital electronics. This sorter is capable of high sorting speeds, simultaneous sorting of 4 distinct populations at once, and real time full matrix compensation. Its current configuration is approved for sorting BSL-2 samples.

Model: 
BD Digital Vantage
Room: 
CCSR 3232
Sort: 
Yes
Maximum cells/second: 
10,000 (35 psi), 4,000 (15 psi)
BSL-2: 
Yes
Colors: 
7
Laser Excitations: 
488nm, 594nm
Verdi

Verdi is a 3-laser, 8-color sorter.  Its current laser/detector configuration provides 2-Blue, 3-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B016C
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
7
Laser Excitations: 
488nm, 561nm, 640 nm

Instrument Specifics

Name Model Room Sort Clone Plate Reader Chiller / Heater Maximum cells / second Minutes to Sort 10[6] Cells BSL-2 Colors Laser Excitations Price per hour with no operator Configuration & Scheduling
Consultation - Main Facility (Beckman Center) B015 $75.00 Consultation - Main Facility (Beckman Center)
Abbott BD LSR II 1651 Page Mill No No Yes 10,000 Yes 17 405nm, 488nm, 633nm $58.00 PDF icon Abbott Configuration
Abbott
Aurora Aurora B016 No No 35000 n/a Yes >20 488, 405, 640 $58.00 Aurora
LSR II BD LSR II B016A No No Yes 10,000 N/A Yes 18 488nm, 532nm, 635nm, 405nm $58.00 PDF icon LSR II Configuration
LSR II
LSR II.2 BD LSR II B016A No No Yes 10,000 N/A Yes 18 488nm, 532nm, 635nm, 405nm, 355nm $58.00 PDF icon LSRII.2 configuration
LSR II.2
LSR II.UV BD LSR II B016D No No Yes Both 10,000 N/A Yes 18 488nm, 561nm, 635nm, 405nm, 355nm $58.00 PDF icon LSRII-UV Configuration
LSR II.UV
Scanford Custom FACScan B016D No No No 3,000 N/A Yes 12 488nm, 405 nm, 561nm, 640nm $40.00 PDF icon Scanford Configuration
Scanford
Cyrano DVS CyTOF II B015A No No No 500 N/A Yes ~100 N/A $140.00 Cyrano
Aida BD Aria II B019 Yes Yes No Both 25,000 4 Yes 13 488nm, 561nm, 635nm, 405nm $70.00 PDF icon Aida Configuration
Aida
Aria BD Aria II B016C Yes Yes No Both 25,000 4 Yes 11 488nm, 635nm, 405nm $70.00 PDF icon Aria Configuration
Aria