Aida
Configuration:
Aida is a 4-laser, 14-color sorter. Its current laser/detector configuration provides 5-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.
Model:
BD Aria II
Room:
B019
Sort:
Yes
Clone:
Yes
Index Sort:
Yes
Maximum cells/second:
25,000
BSL-2:
Yes
Colors:
13
Laser Excitations:
488nm, 561nm, 635nm, 405nm
Minutes to Sort 10[6] Cells:
4
Plate Reader:
No
Chiller/Heater:
Both
Price per hour with no operator:
$70.00
Configuration:
| 405 nm Violet laser 100 mW | |||||||||
| PMT Position | Splitter | BP filter | Fluorochromes | ||||||
| A | 595 | 605/40 | Qdot 605, BV 605 | ||||||
| B | 535 | 560/40 | Qdot 585, Pacific Orange, live/dead Aqua Amine | ||||||
| C | None | 450/50 | Pacific Blue, Alexa 405, Cascade Blue, BV 421, CFP, mCerulean, DyeCycle Violet, DAPI, Hoechst (for live/dead). | ||||||
|
Note: CFP and variants not optimal with 405 nm excitation. Use Carmen for better results. |
|||||||||
| 488 nm Blue laser 50 mW | |||||||||
| PMT Position | Splitter | BP filter | Fluorochromes | ||||||
| A | 685 | 710/50 | PerCP-Cy5.5, PE-Cy5.5 | ||||||
| B | 505 | 525/50 | FITC, GFP, YFP, Venus, Alexa 488, CFSE | ||||||
| C | None | 488/10 | SSC | ||||||
| 561 nm Yellow-Green laser 50 mW | |||||||||
| PMT Position | Splitter | BP filter | Fluorochromes | ||||||
| A | 750 | 780/60 | PE-Cy7 | ||||||
| B | 685 | 710/50 | PE-Cy5.5, 7AAD, PI | ||||||
| C | 635 | 660/20 | PE-Cy5, 7AAD, PI | ||||||
| D | 600 | 610/20 | PE-Texas-Red. mCherry | ||||||
| E | None | 582/15 | PE, tdTomato, DsRed | ||||||
| 640 nm Red laser 40 mW | |||||||||
| PMT Position | Splitter | BP filter | Fluorochromes | ||||||
| A | 750 | 780/60 | APC-Cy7, APC-H7 | ||||||
| B | 690 | 730/45 | APC-Cy5.5, Alexa 700 | ||||||
| C | None | 670/30 | APC, Alexa 647 | ||||||
| Filters: | |||||||||
| LP - Long Pass filter: passes light with wavelength >= number shown. | |||||||||
| SP - Short Pass filter: passes light with wavelength <= number shown. | |||||||||
| BP - Band Pass filter: passes light with wavelength centered on first number and passband second number. | |||||||||
last update 07-09-10
Last modified:
Wed, 12 Sep 2018 at 4:25 pm
