Flow Cytometry Resources

Flow cytometry basics

Introduction to Flow Cytometry from BD Biosciences (must enable Flash)
Introduction to Flow Cytometry – Basics Guide from Bio-Rad
Cytometry 101 from FlowJo University
Fluorescence Tutorials from ThermoFisher
Training Resources from Cheeky Scientist blog

Flow cytometry controls

The Lowdown on Flow Cytometry Experimental Controls from Future Lab blog
Flow Cytometry Controls webinar from CYTO U

Panel design

Optimized Multicolor Immunofluoresence Panels from Cytometry Part A
Panel Design Tool from Chromocyte
CD Marker Expression Profiles from BD Biosciences
FluoroFinder search antibodies from all vendors in one place
Antigen Density for Human and Murine Surface Markers from Bio-Rad

Spectra viewers

FPbase Spectra Viewer easily view spectra of multiple dyes or fluorescent proteins
Cytek Full Spectrum Viewer for spectral panel design
Spectrum Viewer from BD Biosciences
Spectra Viewer from ThermoFisher
Fluorescence Spectra Analyzer from BioLegend

Spectral Flow Cytometry

Introduction to Full Spectrum Flow Cytometry webinar from Memorial Sloan Kettering Cancer Center
Aurora Fluorochrome Selection Guidelines from Cytek

Data analysis software

Getting Started with FlowJo
Getting Started with FCS Express
SpectroFlo Overview
FlowJo Recorded Webinars

Dimensionality reduction and population discovery

FlowJo exchange free downloads of FlowJo plugins
Comparison of clustering methods for high-dimensional single-cell flow and mass cytometry data
The Art of Dimensionality Reduction: What Really Matters webinar from CYTO U
Data Analysis Rigor and Reproducibility, Part 2 – Analysis Tools webinar from CYTO U
FlowSOM FlowJo Plugin Tutorial
FlowSOM: Using self-organizing maps for visualization and interpretation of cytometry data

Specialized applications

Proliferation Monitoring by Flow Cytometry webinar from CYTO U
Apoptosis and Beyond: Cytometry in Studies of Programmed Cell Death
RNA Flow Cytometry Using the Branched DNA Technique
RNA Flow Cytometry webinar from CYTO U
Characterization, detection, and counting of metal nanoparticles using flow cytometry

Cytometry communities

Purdue Flow and Image Cytometry List
CYTO U
Flow Cytometry subreddit