Verdi Fluorochromes

 

561 nm Yellow/Green laser 50 nW            
PMT position Splitter BP filter Fluorochromes        
A 635LP
785/32 PE-Cy7. (PE-Cy5*, PE-Cy55*)        
B 595 LP 616/23

PE-TR, mCherry, dsRed

   
C None 585/15 PE, mOrange
 * BP filter in A can be changed to measure PE-Cy5 or PE-Cy55        
         
488 nm Blue laser 50 mW            
PMT position Splitter BP filter Fluorochromes        
A 635 LP 695/50 PerCP-Cy55        
B 495 LP 530/30 FITC, GFP, Alexa 488, CFSE      
C None 488/10 SSC        
               
640 nm Red laser  40 mW          
PMT position Splitter BP filter Fluorochromes        
A 735 LP 780/60 APC-Cy7        
B 685 LP 710/45 APC-Cy5.5, Alexa 700      
C None 660/40 APC, Alexa 647        
               
Filters:              
LP - Long Pass filter: passes light with wavelength >= number shown.      
SP - Short Pass filter: passes light with wavelength <= number shown.      
BP - Band Pass filter: passes light with wavelength centered on first number and passband second number.  

 

 

 

Updated 07/14/2017