Scanford Fluorochromes

 

NOTE: Red and Violet Lasers are co-linear, which could affect spectral overlaps between fluorochromes measured on either laser.

488 nm Blue 30 mW (all detectors have 560 SP filter in optical path)
PMT Splitter BP filter Possible Fluorochromes
BluFL1 560 SP 525/50 FITC, Alexa 488, GFP, CFSE
BluFL2 640 LP 615/25 PI  
BluFL3 640 LP 710/50 PerCP, PE-Cy5.5, PerCP-Cy5.5
         
640 nm Red 100 mW (all detectors have 645 LP filter in optical path)
PMT Splitter BP filter Possible Fluorochromes
RedFL1 655 LP 661/16 APC, Alexa 647  
RedFL2 655 LP & 680 LP 710/50 APC-Cy5.5, Alexa 700
RedFL3 655 LP & 747 LP 780/60 APC-Cy7,Alexa 750, Alexa 790
         
561 nm Yellow/Green 100 mW (all detectors have 571 LP filter in optical path)
PMT Splitter BP filter Possible Fluorochromes
YelFL1 600 SP 590/20 PE, DsRed, tdTomato,
YelFL2 600 SP 615/25 PE-Texas Red, mCherry
YelFL3 725 SP 690/40 PE-Cy5, PI  
YelFL4 725 SP 740 LP PE-Cy7
Use of YelFL3 for PI recommended when using  FITC or Alexa 488. 
405 nm Violet Laser (Co-linear with Red Laser)
PMT Splitter BP filter Possible Fluorochromes
VolFL1 480 SP 450/50 Pacific Blue, Alexa 405, Brilliant Violet 421,CFP, mCerulean, DyeCycle Violet, DAPI,Cascade Blue
VolFL2 480 SP 545/30 Qdot 525, live/dead Aqua Amine
Filters:      
LP - Long Pass filter: passes light with wavelength >= number shown.
SP - Short Pass filter: passes light with wavelength <= number shown.
BP - Band Pass filter: passes light with wavelength centered on first number and passband second number.

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last update 01-13-14