Panel Design Aides

Designing antibody panels that work sucessfully with your particular experiment can be one of the more difficult challenges in using flow cytometry.

The simplest approach is to plagerize: to use an existing panel which works, and modify it for your needs. The best source for this may be one of your lsb mates.

Cytometry Part A publiishes peer-reviewed Optimized Multicolor Immunofluorescence Panels (OMIP) that are available here. Currently there are over 30 of them, and they can be a very informative starting point for your panel.

If you are going to design one yourself,  a helpful online tool is FluoroFinder, available here. This site has all our instrument configurations, and is a good aid infinding out what conjugations are available commercially.

 

 

 

updated 08/12/2016