Steps To Become a SSFF User

To use the FACS Facility, you will need to do the following:

1. Obtain a SUNetID: The Sunet ID is required for the Facility data management system. For members of the Stanford community, this can be done online. For non-Stanford users, you will need to obtain a sponsored ID. To do this, email the registration form below to SFF Administrator.

2. Registration: Fill out the Registration form. Have it signed by the financially responsible person in your lab (the PI) or company. If you are at Stanford, bring it by the Facility or FAX completed form to: 650-724-1019 or email to Tom Nozaki: nozaki@stanford.edu. If you are from a company FAX completed form AND PURCHASE ORDER to: 650-724-1019 or email to Tom Nozaki: nozaki@stanford.edu

3. On-Line Tutorials & book: The following on-line tutorials prepared by Invitrogen and BD are useful introductions to flow cytometry.

Invitogen Tutorials # 1, 4, 5: These provide a quick introduction to flow cytometry, fluorescence, and data analysis

http://www.invitrogen.com/site/us/en/home/support/Tutorials.html

BD Tutorials: A more detailed introductory flow cytometry course and excellent training for using the DiVa software

http://www.bdbiosciences.com/services/training/elearning/index.jsp

A more complete on-line book (with some oversimplifications) is available at

Flow Cytometry Introduction (from Bio-Rad, PDF 1.3mB)

4. New User Orientation: New User Orientation is offered 2-3 times per month. Participants should complete their Registration prior to attending. There is no charge for attending New User Orientation. To view the schedule for upcoming orientation dates, click here.

The Orientation provides a general introduction to the instrumentation in the Facility, as well as a description of the workflow, from Scheduling to Data Analysis. A review of the few but important policies of the Facility will be presented. There will also be time to answer your questions.  Do not bring samples to Orientation!

5. Operator Support or Self-Operator Training? We offer training to use our analyzers and Aria-class sorters independently. However, if you are going to be sorting or analyzing infrequently, it would be best to request Operator Support from Facility staff, as the complexities of the training require on-going practice to really "stick".  Training sessions can be requested on our online system here:  https://facs.stanford.edu/service  Lead time for training can vary from 1-3 weeks, depending on staff availability.  Operator assist for sorts and analysis can be requested for interim experiments using the same link.

 

 

last update: 08/25/17