Mikado Fluorochromes

Measurement Channels

FL1

FL2

FL3

FL4

FL5

FL6

Bandpass filters

450/50

525/50

600/60

665/30

720/60

785/60

Dyes:

 

 

 

 

 

 

BFP, mCFP (poorly)

 

 

 

 

 

 

DAPI, Hoechst

 

 

 

 

 

 

BV421, AF405, PacBlue

 

 

 

 

 

 

BV510, Live/Dead Aqua, PacOrange

 

 

 

 

 

 

GFP, Venus,YFP, mCitrine

 

 

 

 

 

 

FITC, AF488

 

 

 

 

 

 

CFSE

 

 

 

 

 

 

BV570, BV605

 

 

 

 

 

 

PE, PE-TR, PE-CF594

 

 

 

 

 

 

dsRed, tdTomato, mCherry

 

 

 

 

 

 

BV650

 

 

 

 

 

 

mPlum

 

 

 

 

 

 

7-AAD

 

 

 

 

 

 

PE-Cy5

 

 

 

 

 

 

PerCP-Cy55

 

 

 

 

 

 

BV711

 

 

 

 

 

 

PE-CY55

 

 

 

 

 

 

PE-Cy7

 

 

 

 

 

 

BV785

 

 

 

 

 

 

 

 

 

 

 

 

 

Color blocks indicate laser (violet, blue, or green) for dye maximal excitation. You can measure one reagent from each column’s color blocks.

 

The Bandpass filters are shown as (center wavelength)/(width of passband).

 

 

 

 

 

 

 

 

 

 

 

 

Light Scatter is measured as follows:

 

 

 

 

 

 

FSC (Forward Scatter) with passband filter 488/17

FSC

488/17

 

 

 

 

BSC (Back Scatter - equivalent to SSC) with passband filter 488/17

BSC

488/17

 

 

 

 

 

PDF Version

last update 02-27 -17