LSR II.UV Configuration

355 nm UV laser 20 mW        
PMT position Splitter BP filter Fluorochromes
A 505 LP 525/50 Indo-1 (Green)      
B None 450/50 Indo-1 (Blue), DAPI, Hoechst    
405 nm Violet laser 50 mW
       
PMT position Splitter BP filter Fluorochromes
A 505 LP 525/50 Qdot 525, live/dead Aqua Amine    
B None 450/50 Pacific Blue, Alexa 405, Brilliant Violet 421  
CFP, mCerulean, DyeCycle Violet, DAPI,Cascade Blue
Note: CFP and variants not optimal with 405 nm excitation. Use Carmen for better results.
488 nm Blue laser 50 mW
       
PMT position Splitter BP filter Fluorochromes      
A 685 LP 710/50 PerCP-Cy5.5, PE-Cy5.5    
B 505 LP 525/50 FITC, GFP, Alexa 488, CFSE    
C None 488/10 SSC      
561 nm Green laser 50 mW
       
PMT position Splitter BP filter Fluorochromes      
A 750 LP 780/60 PE-Cy7      
B 685 LP 710/50 PE-Cy5.5, 7AAD, PI    
C 635 LP 660/20 PE-Cy5, 7AAD, PI      
D 600 LP 610/20 PE-Texas-Red. mCherry    
E None 585/15 PE, tdTomato, DsRed    
640 nm Red laser 40 mW
       
PMT position Splitter BP filter Fluorochromes      
A 750 LP 780/60 APC-Cy7      
B 690 LP 730/45 APC-Cy5.5, Alexa 700    
C None 670/30 APC, Alesa 647      
Filters:
LP - Long Pass filter: passes light with wavelength >= number shown.
SP - Short Pass filter: passes light with wavelength <= number shown.
BP - Band Pass filter: passes light with wavelength centered on first number and passband second number.

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last update 11/09/13