LSR II Fluorochromes

 

405 nm Violet laser 50 mW          
PMT position Splitter BP filter Fluorochromes          
A 740 LP 780/60 Qdot 800          
B 670 LP 710/50 Qdot 705          
C 630 LP 660/40 Qdot 655          
D 595 LP 605/40 Qdot 605          
E 570 LP 585/42 Qdot 585          
F 535 LP 560/40 Qdot 565, Pacific Orange        
G 495 LP 515/20 Qdot 525, live/dead Aqua Amine        
H None 450/50 Pacific Blue, Alexa 405, BV 421, CFP*, DyeCycle Violet, DAPI    
           
 Note: CFP and variants not optimal with 405 nm excitation. Use Carmen for better results.      
488 nm Blue laser 50 mW            
PMT position Splitter BP filter Fluorochromes          
A 550 LP 710/50 PerCP-Cy5.5, PE-Cy5.5, For PerCP, change bandpass to 682/33.        
B 505 LP 525/50 + 532 notch FITC, GFP, Alexa 488, CFSE        
C None 488/10 + ND1 SSC          
                 
532 nm Green laser 150 mW          
PMT position Splitter BP filter Fluorochromes          
A 740 LP 780/60 PE-Cy7          
B 690 LP 710/50 PE-Cy5.5, 7AAD, PI        
C 640 LP 670/14 PE-Cy5, 7AAD, PI          
D 600 LP 610/20 PE-Texas-Red. mCherry        
E None 575/25 PE, tdTomato, DsRed        
F None None            
G None None            
H None None            
                 
640 nm Red laser 40 mW            
PMT position Splitter BP filter Fluorochromes          
A 740 LP 780/60 APC-Cy7          
B 685 LP 710/50 APC-Cy5.5, Alexa 700        
C None 670/30 APC, Alexa 647          
                 
Filters:                
LP - Long Pass filter: passes light with wavelength >= number shown.        
SP - Short Pass filter: passes light with wavelength <= number shown.        
BP - Band Pass filter: passes light with wavelength centered on first number and passband second number.    
     

*violet excited fluorescent proteins only works for large copy numbers.

   

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last update 07-09-10