LSR II.2 Fluorochromes

 

405 nm Violet laser 50 mW       
PMT position Splitter BP filter Fluorochromes      
A 735 LP 780/60 Qdot 800      
B 670 LP 685/35 Qdot 705      
C 635 LP 660/60 Qdot 655      
D 595 LP 610/20 Qdot 605      
E 570 LP 585/42 Qdot 585      
F 557 LP 560/20 Qdot 565, Pacific Orange    
G 505 LP 525/50 Qdot 525, live/dead Aqua Amine    
H None 450/50 Pacific Blue, Alexa 405, Cascade Blue, BV 421, CFP, DyeCycle Violet, DAPI
       

Note: CFP and variants not optimal with 405 nm excitation. Use Carmen for better results.


488 nm Blue laser 50 mW       
PMT position Splitter BP filter Fluorochromes      
A 655 LP 710/50 PerCP-Cy5.5, PE-Cy5.5    
B 505 LP 525/50 FITC, GFP, Alexa 488, CFSE    
C None 488/10 SSC      
             
532 nm Green laser 150 mW       
PMT position Splitter BP filter Fluorochromes      
A 735 LP 780/60 PE-Cy7      
B 685 LP 710/50 PE-Cy5.5, 7AAD, PI    
C 635 LP 660/20 PE-Cy5, 7AAD, PI      
D 600 LP 610/20 PE-Texas-Red. mCherry    
E 570 LP 585/15 PE, tdTomato, DsRed    
             
640 nm Red laser 40 mW        
PMT position Splitter BP filter Fluorochromes      
A 735 LP 780/60 APC-Cy7      
B 685 LP 730/45 APC-Cy5.5, Alexa 700    
C None 670/30 APC, Alexa 647      
             
Filters:            
LP - Long Pass filter: passes light with wavelength >= number shown.    
SP - Short Pass filter: passes light with wavelength <= number shown.    
BP - Band Pass filter: passes light with wavelength centered on first number and passband second number.

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last update 07-20-10