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Available Instruments

Analyzers

Abbott is a 3-laser, 17-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 8-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
1651 Page Mill
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
405nm, 488nm, 633nm

Abbott is a 3-laser, 17-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 8-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD LSR ll
Room: 
1651 Page Mill
Maximum cells/second: 
10000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
405nm, 488nm, 633nm

3-laser (Blue-Violet-Red) spectral cytometer capable of  analyzing >20-color panels

Model: 
Aurora
Room: 
B016
Sort: 
No
Maximum cells/second: 
35000
BSL-2: 
Yes
Colors: 
>20
Laser Excitations: 
488, 405, 640

5-laser (UltraViolet-Blue-Violet-Yello-Red) spectral cytometer capable of  analyzing >30-color panels

Model: 
Aurora
Room: 
B016
Sort: 
No
Maximum cells/second: 
3500
BSL-2: 
Yes
Colors: 
>35
Laser Excitations: 
355 405 488 561 640

LSRII is a 4-laser, 18-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 8-Violet, 2-Blue, 5-Green, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
B016A
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 532nm, 635nm, 405nm

LSRII.2 is a 5-laser, 18-color instrument compatible with the 96-well plate sampler.  Its current laser/detector configuration provides 6-Violet, 2-Blue, 4-Green, 3-UV, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
B016A
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm

LSRII.UV is a 5-laser, 18-color instrument compatible with the 96-well plate sampler.  It has recently been upgraded to support the latest violet and UV excited reagents.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 5-Yellow, and 3-Red laser excited detection channels.

Model: 
BD LSR II
Room: 
B016D
Sort: 
No
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm

4-laser, 25-fluorescent channels plus 2 scatter channels; excellent plate loader for both 96- and 384-well plates or up to 40 sample tubes

Model: 
Acea Novocyte Quanteon
Room: 
B016C
Sort: 
No
Maximum cells/second: 
20000
BSL-2: 
Yes
Colors: 
25
Laser Excitations: 
Blue, Violet, Yellow, Red

4-laser, 25-fluorescent channels plus 2 scatter channels; excellent plate loader for both 96- and 384-well plates or up to 40 sample tubes

Model: 
Acea Novocyte Quanteon
Room: 
B003
Sort: 
No
Maximum cells/second: 
20000
BSL-2: 
Yes
Colors: 
25
Laser Excitations: 
Blue, Violet, Yellow, Red

The FACScan Analyzer was one of the first successful bench top flow cytometers. They just keep going and going. This one has been upgraded to 4-laser, 12-color configuration.  Its current laser/detector configuration provides 2-Violet(co-linear with Red, 3-Red (co-linear with Violet), 3-Blue, and 4-Yellow laser excited detection channels.

Model: 
Custom FACScan
Room: 
B016D
Sort: 
No
Maximum cells/second: 
3,000
BSL-2: 
Yes
Colors: 
12
Laser Excitations: 
488nm, 405 nm, 561nm, 640nm

BD FACS Symphony A5 is a  5-laser, 29-color instrument compatible with the 96-well plate sampler.  

Model: 
BD FACS Symphony A5
Room: 
B003
Sort: 
No
Maximum cells/second: 
10000
Colors: 
29
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm

Mass Cytometer

The CyTOF2 Mass Cytometer uses metal-ion conjugates to label cells.  Labeled cells are then ionized and mass measurements are used to differentiate functional and phenotypic markers.  Sample preparation and processing is quite similar to that used in fluorescence-based cytometry with some quality control considerations unique to atomic mass spectroscopy. Please contact staff for further details.

Model: 
DVS CyTOF II
Room: 
B015A
Sort: 
No
Maximum cells/second: 
500
BSL-2: 
Yes
Colors: 
~100
Laser Excitations: 
N/A

The CyTOF2 Mass Cytometer uses metal-ion conjugates to label cells.  Labeled cells are then ionized and mass measurements are used to differentiate functional and phenotypic markers.  Sample preparation and processing is quite similar to that used in fluorescence-based cytometry with some quality control considerations unique to atomic mass spectroscopy. Please contact staff for further details.

Model: 
DVS CyTOF ll
Room: 
B015A
Sort: 
No
Maximum cells/second: 
500
BSL-2: 
Yes
Colors: 
~100
Laser Excitations: 
N/A

Sorters

Aida

Aida is a 4-laser, 14-color sorter.  Its current laser/detector configuration provides 5-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
13
Laser Excitations: 
488nm, 561nm, 635nm, 405nm
Aria

Aria is a 3-laser, 11-color sorter.  Its current laser/detector configuration provides 3-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B016C
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
11
Laser Excitations: 
488nm, 635nm, 405nm
Aria

Aria is a 3-laser, 11-color sorter.  Its current laser/detector configuration provides 3-Violet, 6-Blue, and 3-Red laser excited detection channels.

Model: 
BD Aria ll
Room: 
B016C
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
11
Laser Excitations: 
488nm, 635nm, 405nm
Carmen

Carmen is a 6 laser sorter capable of up to 6 way sorting.  It is our fastest plate sorter; both 384- and 96- well plate sorts require only 1/3 the time needed on an Aria type sorter.  It is the only instrument with an 457nm excitation laser, making it ideal for fluorochromes such as CFP or mTurquoise.  It is available only with operator support.

Model: 
BD Influx Special Order
Room: 
B015A
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
355nm, 405nm, 457nm, 488nm, 561nm, 640nm

Costello is a 5 laser, 18-color Aria Fusion sorter.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria Fusion
Room: 
1651 Page Mill
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm

Costello is a 5 laser, 18-color Aria Fusion sorter.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria Fusion
Room: 
1651 Page Mill
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm
Falstaff

Falstaff is a 5-laser, 17-color sorter.  Its current laser/detector configuration provides 2-UV, 5-Violet, 2-Blue, 5-Green, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm

Falstaff is a 5-laser, 17-color sorter.  Its current laser/detector configuration provides 2-UV, 5-Violet, 2-Blue, 5-Green, and 3-Red laser excited detection channels.

Model: 
BD Aria ll
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
17
Laser Excitations: 
488nm, 532nm, 635nm, 405nm, 355nm
Mikado

is the most automated sorter that the Facility has. It is simple to use, and has tray and idex sorting. It is the best sorter for 384 trays, It has 3 lasers (405 nm, 488nm, 561nm) but is a little limited in fluorochrome choices as these lasers are co-linear,

Model: 
Sony SH800s
Room: 
B016B
Sort: 
Yes
Maximum cells/second: 
7500
BSL-2: 
Yes
Colors: 
6
Laser Excitations: 
405nm,488nm,561 nm

is the most automated sorter that the Facility has. It is simple to use, and has tray and idex sorting. It is the best sorter for 384 trays, It has 3 lasers (405 nm, 488nm, 561nm) but is a little limited in fluorochrome choices as these lasers are co-linear

Model: 
Sony SH800s
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
7500
BSL-2: 
Yes
Colors: 
6
Laser Excitations: 
405nm,488nm,561 nm

Book a 30 min slot ahead of your sort for staff to start-up the instrument

FREE of charge

You MUST provide the nozzle size needed for the experiment

Model: 
Sony
Room: 
B016
PICI

PICI is a 5 laser, 18 color Aria Fusion sorter that was bought with Parker Institute for Cancer Immunotherapy funds.  Its current laser/detector configuration provides 3-UV, 6-Violet, 2-Blue, 4-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria Fusion
Room: 
B019
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
18
Laser Excitations: 
488nm, 561nm, 635nm, 405nm, 355nm
Satchmo

is a simple two-laser (488 nm and 640 nm) sorter. It is easy to operate, and does fast tray sorting, as well as index sorting. It uses the same polypropylene sample tubes as Carmen.

Model: 
BD FACSJazz
Room: 
B016B
Sort: 
Yes
Maximum cells/second: 
10,000
BSL-2: 
Yes
Colors: 
6
Laser Excitations: 
488 nm, 640 nm
Verdi

Verdi is a 3-laser, 8-color sorter.  Its current laser/detector configuration provides 2-Blue, 3-Yellow, and 3-Red laser excited detection channels.

Model: 
BD Aria II
Room: 
B016C
Sort: 
Yes
Maximum cells/second: 
25,000
BSL-2: 
Yes
Colors: 
7
Laser Excitations: 
488nm, 561nm, 640 nm

Instrument Specifics

Name Model Room Sort Clone Plate Reader Chiller / Heatersort descending Maximum cells / second Minutes to Sort 10[6] Cells BSL-2 Colors Laser Excitations Price per hour with no operator Configuration & Scheduling
Aurora Aurora B016 No 3500 n/a Yes >35 355 405 488 561 640 $40.00 Aurora
Cyrano DVS CyTOF II B015A No No No 500 N/A Yes ~100 N/A $140.00 Cyrano
Quanteon Sample Drop-off Acea Novocyte Quanteon B003 No Yes 20000 Yes 25 Blue, Violet, Yellow, Red $40.00 PDF icon Quanteon Configuration
Quanteon Sample Drop-off
LSR II BD LSR II B016A No No Yes 10,000 N/A Yes 18 488nm, 532nm, 635nm, 405nm $58.00 PDF icon LSR II Configuration
LSR II
LSR II.2 BD LSR II B016A No No Yes 10,000 N/A Yes 18 488nm, 532nm, 635nm, 405nm, 355nm $58.00 PDF icon LSRII.2 configuration
LSR II.2
Cyrano Request Only DVS CyTOF ll B015A No No No 500 N/A Yes ~100 N/A $140.00 Cyrano Request Only
Symphony BD FACS Symphony A5 B003 No No Yes 10000 29 488nm, 532nm, 635nm, 405nm, 355nm $58.00 Symphony
Scanford Custom FACScan B016D No No No 3,000 N/A Yes 12 488nm, 405 nm, 561nm, 640nm $40.00 PDF icon Scanford Configuration
Scanford
Abbott BD LSR II 1651 Page Mill No No Yes 10,000 Yes 17 405nm, 488nm, 633nm $58.00 PDF icon Abbott Configuration
Abbott
Aurora Aurora B016 No No 35000 n/a Yes >20 488, 405, 640 $58.00 Aurora