Falstaff Fluorochromes


355 nm UV laser 60 mW            
PMT position Splitter BP filter Fluorochromes        
A 635 670/50 Hoechst Red        
B None 450/50 Hoechst Blue, DAPI      
               
405 nm Violet laser 100 mW            
PMT position Splitter BP filter Fluorochromes        
A 630 660/20 Qdot 655,BV650        
B 595 605/40 Qdot 605, BV605        
C 545 560/40 Qdot 565, Pacific Orange      
D 505 525/50 Qdot 525, live/ded Aqua Amine, AmCyan    
E None 450/50 Pacific Blue, Alexa 405, Cascade Blue, BV421, CFP, mCerulean, DyeCycle Violet  
         
Note: CFP and variants not optimal with 405 nm excitation. Use Carmen for better results.

488 nm Blue laser 50 mW            
PMT position Splitter BP filter Fluorochromes        
A 685 710/50 PerCP-Cy5.5, PE-Cy5.5      
B 505 525/50 FITC, GFP, Alexa 488, CFSE      
C None 488/10 SSC        
               
532 nm Green laser 150 mW            
PMT position Splitter BP filter Fluorochromes        
A 750 780/60 PE-Cy7        
B 685 710/50 PE-Cy5.5, 7AAD, PI      
C 635 660/20 PE-Cy5, 7AAD, PI        
D 600 610/20 PE-Texas-Red. mCherry      
E None 575/25 PE, tdTomato, DsRed      
               
640 nm Red laser 40 mW            
PMT position Splitter BP filter Fluorochromes        
A 750 780/60 ACP-Cy7        
B 690 730/45 APC-Cy5.5, Alexa 700      
C None 670/30 APC, Alexa 647        
               
Filters:              
LP - Long Pass filter: passes light with wavelength >= number shown.      
SP - Short Pass filter: passes light with wavelength <= number shown.      
BP - Band Pass filter: passes light with wavelength centered on first number and passband second number.  

 

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last update 07-09-10