Costello Fluorochromes

355 nm UV laser 15 mW

 

 

 

 

PMT

Splitter

BP filter

Fluorochromes

 

 

A

690LP

740/30

BUV737

 

 

 

B

505LP

515/30

BUV496, Hoechst, DAPI

 

 

C

None

379/28

BUV395

 

 

 

 

 

 

 

 

 

 

405 nm Violet laser 100 mW

 

 

 

PMT

Splitter

BP filter

Fluorochromes

 

 

A

750LP

780/60

BV786

 

 

 

B

690LP

710/50

BV711

 

 

 

C

635LP

670/30

BV650

 

 

 

D

595LP

610/20

BV605

 

 

 

E

505LP

525/50

BV510, Pacific Orange, live/dead Aqua Amine

F

None

450/50

BV421, Pacific Blue, Alexa 405, Cascade Blue

 

 

 

CFP, BFP, mCerulean, DyeCycle Violet, DAPI, Hoechst (for live/dead)

Note: CFP and variants not optimal with 405 nm excitation. Use Carmen for better results.

 

 

 

 

 

 

 

488 nm Blue laser 100 mW

 

 

 

PMT

Splitter

BP filter

Fluorochromes

 

 

A

635LP

695/40

PerCP-Cy5.5, PE-Cy5.5

 

 

B

505

525/50

FITC, GFP,YFP, Venus, Alexa 488, CFSE

 

C

None

488/10

SSC

 

 

 

 

 

 

 

 

 

 

561 nm Green laser 100 mW

 

 

 

PMT

Splitter

BP filter

Fluorochromes

 

 

A

750LP

780/60

PE-Cy7

 

 

 

B

635LP

670/30

PE-Cy5, 7AAD, PI, mKate

 

 

C

595LP

619/20

mCherry, PE-TR, PE-CF594

 

 

D

None

582/15

PE, tdTomato, DsRed

 

 

 

 

 

 

 

 

 

640 nm Red laser 140 mW

 

 

 

PMT

Splitter

BP filter

Fluorochromes

 

 

A

750LP

780/60

APC-Cy7

 

 

 

B

685LP

730/45

APC-Cy5.5, Alexa 700

 

 

C

None

670/30

APC, Alexa 647, iRFP

 

 

 

 

 

 

 

 

 

Filters:

 

 

 

 

 

LP - Long Pass filter: passes light with wavelength >= number shown.

SP - Short Pass filter: passes light with wavelength <= number shown.

BP - Band Pass filter: passes light with wavelength centered on first number and passband second number.

 

 

 

updated 02-29-17