Carmen Fluorochromes

355 nm UV Laser 100 mW
PMT Position BP Filter Fluorochromes
10 460/50 Hoechst Blue, DAPI (DNA), Indo-1 Blue*
11 530/30 Indo-1 Green, Hoechst Red*
*Filter change necaessary.
405 nm Violet Laser 50 mW
PMT Position BP Filter Fluorochromes
15 460/50 Pacific Blue, BV-421, Alexa 405, Cascade Blue
DyeCycle Violet, DAPI (live/dead)
21 520/20 BV-510, Aqua Fixable Live/Dead
22 620/20 BV-605, Qdot 605
457 nm Short Blue Laser 300 mW
PMT Position BP Filter Fluorochromes
12 480/40 CFP, mCerulean, mTurquoise
13 550/50 Chromomycin-A3
488 nm Blue Laser 200 mW
PMT Position BP Filter Fluorochromes
1 488/10 Forward Scatter (FSc)
2 488/10

Side Scatter (SSC)

3 525/50 FITC, Alexa 488, GFP, YFP, mCitrine, mVenus
4 692/40 PerCp-Cy5.5, PI (live/dead)
532 nm Green Laser 150 mW
PMT Position BP Filter Fluorochromes
5 585/29 PE, dsRed
6 610/20 PE-TR PE-CF594, mCherry, dtTomato, RFP variants
8 670/30 PE-Cy5, 7-AAD, PI (DNA)
9 750 LP PE-Cy7
561 nm Yellow-Green Laser 75 mW
PMT Position BP Filter Fluorochromes
16 610/20 PE-TR PE-CF594, mCherry, dtTomato, RFP variants
The 561 laser can be moved to the 532 laser pinholes for more effiecient excitation of PE and tandems.
640 nm Red Laser 50 mW
PMT Position BP Filter Fluorochromes
14 670/30 APC, Alexa 647
19 730/40 APC-Cy5.5, Alexa 700
20 750 LP APC-Cy7, APC-H7
LP = Long Pass Filter

 

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updagted 01/17/14