Aria Fluorochromes

 

405 nm Violet laser            
PMT position Splitter BP filter Fluorochromes        
A 595 LP 605/40 Qdot 605, BV 605        
B 502 LP 540/80 Qdot 585, Pacific Orange, live/dead Aqua Amine    
C None 450/50 Pacific Blue, Alexa 405, Cascade Blue, BV 421, CFP*, mCerulean*, DyeCycle Violet, DAPI, Hoechst (for live/dead)  
 * Only works for high expression levels.        
Note: CFP and variants not optimal with 405 nm excitation. Use Carmen for better results. 
488 nm Blue laser 60 mW            
PMT position Splitter BP filter Fluorochromes        
A 735 LP 780/60 PE-Cy7        
B 685 LP 710/40 PE-Cy5.5, PerCP-Cy5.5, 7AAD, PI      
C 635 LP 665/30 PE-Cy5, 7AAD, PI        
D 595 LP 610/20 PE-Texas-Red        
E 556 LP 575/26 PE, DsRed (poor)        
F 502 LP 525/50 FITC, GFP, Alexa 488, CFSE      
G None 488/10 SSC        
               
640 nm Red laser  15 mW          
PMT position Splitter BP filter Fluorochromes        
A 735 LP 780/60 APC-Cy7        
B 685 LP 710/45 APC-Cy5.5, Alexa 700      
C None 660/40 APC, Alexa 647        
               
Filters:              
LP - Long Pass filter: passes light with wavelength >= number shown.      
SP - Short Pass filter: passes light with wavelength <= number shown.      
BP - Band Pass filter: passes light with wavelength centered on first number and passband second number.  

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last update 07-20-10