Aida Fluorochromes

405 nm Violet laser 100 mW          
PMT Position Splitter BP filter Fluorochromes      
A 595 605/40 Qdot 605, BV 605      
B 535 560/40 Qdot 585, Pacific Orange, live/dead Aqua Amine  
C None 450/50 Pacific Blue, Alexa 405, Cascade Blue, BV 421, CFP, mCerulean, DyeCycle Violet, DAPI, Hoechst (for live/dead).
       

Note: CFP and variants not optimal with 405 nm excitation. Use Carmen for better results.     

488 nm Blue laser 50 mW         
PMT Position Splitter BP filter Fluorochromes      
A 685 710/50 PerCP-Cy5.5, PE-Cy5.5    
B 505 525/50 FITC, GFP, YFP, Venus, Alexa 488, CFSE    
C None 488/10 SSC      
             
561 nm Yellow-Green laser 50 mW       
PMT Position Splitter BP filter Fluorochromes      
A 750 780/60 PE-Cy7      
B 685 710/50 PE-Cy5.5, 7AAD, PI    
C 635 660/20 PE-Cy5, 7AAD, PI      
D 600 610/20 PE-Texas-Red. mCherry    
E None 582/15 PE, tdTomato, DsRed    
             
640 nm Red laser 40 mW       
PMT Position Splitter BP filter Fluorochromes      
A 750 780/60 APC-Cy7, APC-H7      
B 690 730/45 APC-Cy5.5, Alexa 700    
C None 670/30 APC, Alexa 647      
             
Filters:          
LP - Long Pass filter: passes light with wavelength >= number shown.    
SP - Short Pass filter: passes light with wavelength <= number shown.    
BP - Band Pass filter: passes light with wavelength centered on first number and passband second number.

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last update 07-09-10